In addition, 7-DHC treatment promoted the proliferation of VDR KO KGN cells and increased ovarian reserve-related mRNA expression of Amh and Amhr2; and estrogen synthesis-related mRNA levels of Hsd17b1 and Cyp19a1. Furthermore, a study has shown that weaned pups of Vdr deficiency mice to a high calcium-containing diet could restore their fertility, whether calcium metabolism affects granulosa cell activity and the effect of calcium on ovarian development deserves further study (Johnson and DeLuca, 2001). The gene discussed is VDR; the disease is hyperinsulinemic hypoglycemia, familial, 4.