Then, single-cell sequencing revealed that CD248 is co-expressed on tumor and stromal cells within the TME in various cancers, including OSCC (Figures 9C, D), ESCC (Figures 9E, F), HSCC (Figures 9G, H), BRCA (Figures 9I, J), PDAC (Figures 9K, L), PAAD (Supplementary Figures S6A, B), GBM (Supplementary Figures S6C, D), CRC (Supplementary Figures S6E, F), and SKCM (Supplementary Figures S6G, H). The gene discussed is CD248; the disease is esophageal squamous cell carcinoma.