NOS1 and Infertility: Humans and mice with loss-of-function mutations in nNOS (encoded by NOS1 and Nos1 genes, respectively) are infertile and global inhibition or deletion of NO production precludes leptin-induced LH secretion (Yu et al., 1997; Gyurko et al., 2002; Bellefontaine et al., 2014; Chachlaki et al., 2022).