Whereas lack of LepR in glutamatergic neurons did not disrupt the reproductive function (Zuure et al., 2013; Martin et al., 2014), female mice lacking vesicular glutamate transporter 2 (Vglut2) in LepR neurons developed late-onset obesity, altered pubertal maturation, disrupted estrous cycles, and mild subfertility (Sáenz de Miera et al., 2024). This evidence concerns the gene LEPR and obesity due to melanocortin 4 receptor deficiency.