Higher serum ANGPTL8 levels were present in the moderate-to-severe NAFLD group compared to the non-NAFLD and mild NAFLD groups (1,129 ± 351 vs 742 ± 252 and 765 ± 301 pg/mL, respectively, p = 0.001). ANGPTL8 levels were comparable between the non-NAFLD and mild NAFLD groups. Here, ANGPTL8 is linked to metabolic dysfunction-associated steatotic liver disease.