To investigate the effects of ARF6 knockdown, HL‐60 and MV‐4‐11 AML cell lines were transfected with four various siRNAs targeting ARF6 (si‐1‐584, ‐2‐803, ‐3‐895, ‐4‐1051). This evidence concerns the gene ARF6 and acute myeloid leukemia.