APOE and Alzheimer disease: DEG analysis comparing astrocytes of 5w Aβ vs. ctrl hiNS(+), to examine chronic Aβ-induced transcriptional changes exclusively in the presence of hiMG, highlighted significant upregulation of a number of genes associated with reactive astrocytes in human AD: 1) LRP1 is a receptor for APOE and has been associated with neuroprotection in animal models of AD [95, 96], 2) CLU is an extracellular chaperone linked to neuroprotection via Aβ aggregation and clearance by astrocytes [97, 98], and 3) VIM was reported to be upregulated in AD although its role in pathology remains elusive [99–101].