The expression of AD-associated astrocyte genes depends on the presence of hiMG during Aβ exposure, including genes such as GFAP, APOE, and VIM. Interestingly, we found DEGs linked to oxidative stress handling (OXR1, CCL2 and CHL1) to be upregulated only in 3w Aβ hiNS(+), the experimental group in which we observed a significant reduction in Aβ induced oxidative stress (Fig. 5B). Here, CHL1 is linked to Alzheimer disease.