When analyzing the transcriptional profile of hiMG, we found that hiMG in 5w Aβ hiMG(+) sub-cluster into three distinct populations (Fig. 7) including one group resembling a unique transcriptional profile of disease associated microglia [50] which includes the expression of APOE and TREM2, two genes extensively studied in AD research. The gene discussed is TREM2; the disease is Alzheimer disease.