GUSB and mucopolysaccharidosis type 7: The data obtained by RT‒qPCR analysis of the cDNA levels of the GUSB gene in both groups indicated that the patient with MPS VII had a higher level of expression (Log102(−ΔCT) = − 2.04822) than did the control group, which presented a mean differential expression of − 2.219019 and a standard deviation of 0.2 (Fig. 4A).