To further validate the roles of PPARα in antidepressant effect of FMN, we treated PPARα antagonist GW6471 along with FMN (40 mg/kg, the more effective dose) in LPS-induced depressive mice, then carried out SPT, OFT, and TST to assess depression-like behaviors (Fig. 7A). This evidence concerns the gene PPARA and depressive symptom measurement.