BDH1 and steatosis: Maintenance of independent cohorts of BDH1-Liver-KO and littermate control mice on a 60% kcal high-fat diet (HFD) for 16 weeks also showed no evidence of increased steatosis, inflammation, or fibrosis — with a trend toward a diminution in fibrosis in livers of BDH1-Liver-KO mice (56% ± 30%, Student’s t test, P = 0.06) (Supplemental Figure 10, A–C).