First, the current WHO categorization of SIL is based on the thickness of dysplastic changes in epithelium.5 In addition, several previous studies have evaluated different markers according to the thickness of staining in squamous epithelium and specifically, p16 positivity in cervical SIL is defined as continuous staining in at least basal third of the epithelium.6,29,40 Another strength of our study is objective evaluation system for both NANOG and SOX2. Here, SOX2 is linked to cervical squamous intraepithelial neoplasia.