To assess the role of muscle‐derived sEVs in cognitive protection in CCH rats, we developed an adenoviral vector system encoding Rab27a shRNA to silence Rab27a expression, a known regulator of EV biogenesis and secretion.[56, 57] CCH rats received bilateral intramuscular injections of AAV‐Rab27a‐shRNA to inhibit muscle‐derived sEV secretion (Figure 6B), and successful infection of muscle cells was confirmed (Figure S7H, Supporting Information). The gene discussed is RAB27A; the disease is infection.