TP53 and bone neoplasm: In bone tumor research, the common genetic mutations investigated include mutations in tumor suppressor genes like p53 or oncogenes like Ras. The CRISPR-Cas9 has been used to create p53 knockout OS cell lines, revealing that, although the p53 mutation can inhibit tumor cell growth, it cannot completely prevent tumor invasion and metastasis (Shimizu et al., 2022).