In contrast, at 98 dpi, TMEV-infected Glb1-/- mice displayed an increase in the Iba1-positive area compared to TMEV-infected Glb1+/+ mice indicating a long-lasting effect of TMEV infection on the number and/or size of microglia/macrophages in the brain (maybe due to a transition from a ramified to a hyperramified or amoeboid state of microglial cells), which seems to be enhanced by GM1 lysosomal storage (Figure 6C1-D5). This evidence concerns the gene GLB1 and infection.