To elucidate the role of PTBP3 in gastric cancer tumor cells, we transduced organoids with lentiviral vectors to establish PTBP3‐overexpressing models in organoids derived from primary gastric cancer tissues (P‐PDO) and PTBP3‐knockdown models in organoids derived from peritoneal metastatic gastric cancer tissues (M‐PDO). This evidence concerns the gene PTBP3 and gastric cancer.