TP53 and Hyperglycemia: To determine whether hyperglycemia can stabilize p53 expression, Western blotting was performed, revealing that treatment with cycloheximide (CHX) protein synthesis inhibitor) in the presence of either MG-132 (proteasome-mediated degradation inhibitor) or hyperglycemia-stabilized phosphorylated p53 due to its inefficient degradation (Fig. 1G).