Agarose gel electrophoresis of PCR products, using primers covering exons 1 and 6 of human ULK1 mRNA, showed a newly generated shorter transcript of approximately 200 bp in TTK knockdown BC cells (Fig. 6D and Supplementary Fig. S6E), with no significant differences observed in other regions (Supplementary Fig. S6F). This evidence concerns the gene ULK1 and breast cancer.