CYP8B1 and metabolic disease: Upon entering the intestine, these conjugated bile acids are deconjugated by gut microbiota.69 In the bile acid synthetic pathway, a significant alteration in the ratios of 12-OH to non-12-OH bile acids and conjugated to unconjugated bile acids are considered as biomarkers of metabolic diseases.70 Bile acids metabolism disorders are closely related to high-fat-induced hepatic lipid accumulation, accompanied by a significant up-regulation of CYP8B1 mRNA in the liver,61,71 consistent with our findings.