To elucidate the protective mechanism of EEPD1 knockout on endothelial cells in the context of atherosclerosis, we conducted high‐throughput RNA sequencing (RNA‐seq) across four experimental groups: siCon, siCon+TNFα, siEEPD1, and siEEPD1+TNFα. The gene discussed is EEPD1; the disease is atherosclerosis.