The expression of TM9SF4 in the T3-4 stages, poor differentiation grade and TNM stage III-IV was considerably greater than that in the T1-2, well differentiation grade and TNM I-II stage (P < 0.05), suggesting that high TM9SF4 expression was strongly connected to the incidence, development, metastasis and invasion in HCC. Here, TM9SF4 is linked to hepatocellular carcinoma.