Analyzing the levels of pro-inflammatory cytokine synthesis in the liver by Western blotting (Figure 3G), in the case of IL-1β, two-way ANOVA analysis revealed significant effects of obesity (F (1, 8) = 8.935; p = 0.0174) and OEA-DS administration (F (1, 8) = 7.157; p = 0.0281), but not their interactions. The gene discussed is IL1B; the disease is obesity due to melanocortin 4 receptor deficiency.