For TNFα, two-way ANOVA revealed significant effects of obesity (F (1, 8) = 14.87; p = 0.0048), OEA-DS administration (F (1, 8) = 6.188; p = 0.0377), and the interaction of these factors (F (1, 8) = 15.03; p = 0.0047). This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.