When assessing the area of distribution of Iba-1-positive macrophages, two-way ANOVA revealed a significant effect of obesity (F (1, 75) = 83.05; p < 0.0001), OEA-DS administration (F (1, 75) = 52.75; p < 0.0001), and their interaction (F (1, 75) = 83.91; p < 0.0001) (Figure 3B). This evidence concerns the gene AIF1 and obesity due to melanocortin 4 receptor deficiency.