Immuno-PET imaging in an orthotopic mouse glioma model showed significant uptake of 89Zr-DFO-OX40 mAb in the spleen, tumour-draining LNs (cervical) and LNs near the vaccinated site (axillary) of mice treated with a cocktail of CpG-oligonucleotides, tumour lysates, and OX40 mAbs compared to controls. This evidence concerns the gene TNFRSF4 and neoplasm.