This method has already beensuccessfully used to generate isogenic cell lines to model anumber of diseases, namely Alzheimer’s disease (APOE4gene sequence correction), sickle cell anaemia (β-globingene), Hutchinson–Gilford progeria (lamin A gene), hereditaryhaemochromatosis (HFE gene) and some cancers (TP53 gene)(Komor et al., 2016; Gaudelli et al., 2017; Koblan et al., 2021;Newby et al., 2021). The gene discussed is HFE; the disease is early-onset autosomal dominant Alzheimer disease.