• Validation of 10 key genes, including KIF20A, NUF2, TTK, TPX2, TOP2A, CDC6, DLGAP5, NCAPG, CCNB1, and KIF11, associated with non-small cell lung cancer• Exploring herbal composition to herbal medicine prediction based on improved algorithms. The gene discussed is CCNB1; the disease is non-small cell lung carcinoma.