AKT1 and infection: In this study, we reveal that: (1) WSSV infection significantly activates the metabolic flux of de novo nucleotide synthesis pathways; (2) Glutamine, rather than glucose, is preferentially utilized to drive purine and pyrimidine synthesis; (3) WSSV enhances both the expression and activity of key enzymes involved in de novo nucleotide metabolism; (4) de novo nucleotide synthesis is critical for WSSV replication; and (5) The Ras-PI3K-AKT-mTOR signaling pathway is not the primary regulatory mechanism for this activation, suggesting the involvement of alternative pathways.