Immunohistochemical staining revealed that treatment of the 4T1 tumor with adenoviral SDCBP shRNA significantly decreased the expression levels of SDCBP, BACH1, and Ki67, but increased the expression levels of NDUFA4 and COX6B2 compared with those in the control and metformin-treated groups (Fig. 6G). This evidence concerns the gene COX6B2 and neoplasm.