We wanted to understand whether gradual reduction of RAB23 in human osteosarcoma cells (MG-63) shows the reduced phenomenon of transferrin uptake that showed in mouse calvaria derived Rab23-/- cells and whether it is possible to normalize transferrin uptake by overexpressing RAB23 in RAB23 knockdown MG-63 cells. Here, RAB23 is linked to osteosarcoma.