SPOP and posterior cortical atrophy: At the 24-hour time point, the remaining activated Ku70 foci and BRCA1 foci may indicate a delay in the overall DDR process in the PIAS1-knockdown group (Supplemental Figure 3, F and G), indicating that PIAS1 maintains genomic stability by promoting HR while inhibiting NHEJ to facilitate DDR, similar to the reported roles of SPOP in the PCa DDR process (6, 7).