We examined the expression of TBRG4 across various cancer types and found it to be significantly upregulated in tumor tissues compared to normal controls in bladder cancer, breast cancer, cervical cancer, bile duct cancer, colon cancer, esophageal cancer, glioblastoma, head and neck cancer, kidney chromophobe, kidney clear cell carcinoma, kidney papillary cell carcinoma, lower-grade glioma, liver cancer, LUAD, lung squamous cell carcinoma, pancreatic cancer, rectal cancer, melanoma, stomach cancer, testicular cancer, endometrioid cancer, and uterine carcinosarcoma. This evidence concerns the gene TBRG4 and malignant colon neoplasm.