In a univariate analysis of generalized linear mixed-effects model, gender, age, time, baseline CD4, weight, disclosure, educational level, occupation, adherence, WHO stage, social support, TB status, opportunistic infection, body mass index, baseline viral load, functional status, and the interaction effect of follow-up time, and educational level variables are significant at 25% level of significance for CD4 cell count. This evidence concerns the gene CD4 and Opportunistic infection.