Immunoblot analysis also shows that TGF‐β‐induced upregulation of EMT‐associated molecules, including N‐cadherin, Snail, Twist1, and ZEB1, was abrogated by ProT overexpression in A549 (Fig. 2C) and H1299 (Fig. 2D) human lung cancer cells. The gene discussed is TWIST1; the disease is lung cancer.