CD4 and neoplasm: Next, we investigated the correlation between changes in IL-35 copy number and the abundance of immune cells that play a major role in anti-tumor immunity, such as B cells, CD8+ T cells, CD4+ T cells, macrophage, neutrophil cells, and dendritic cells, in LUAD and lung squamous carcinoma (LUSC), and showed a decrease in immune infiltration compared to normal when the gene encoding IL-35 was amplified, especially in CD8+ T cells, CD4+ T cells and DC cells (Fig. 3E), suggesting that IL-35 is an important target for immune evasion.