We further investigated the effect of high expression of IL-35-related FXR1 and ACTR3 on tumor microenvironment (TME) and found that ACTR3 was significantly associated with EMT signatures and FXR1 was positively associated with NSCLC signatures, which were significantly and positively correlated (Fig. 4E). Here, FXR1 is linked to non-small cell lung carcinoma.