Research indicates that the diagnostic effectiveness of ultrasound screening for detecting early HCC reaches 45% while combined serum AFP testing only reaches 63% success rate.[5] In addition, although tissue biopsy can accurately diagnose HCC, it is an invasive diagnostic method that may increase needle metastasis.[6] The detection methods for earlier diagnosis of HCC have been expanding to include decarboxylated prothrombin (DCP) alongside glypican-3 and bone bridge protein as potential serum biomarkers. This evidence concerns the gene ACE and hepatocellular carcinoma.