GO and KEGG analyses revealed that pathways in cancer, lipid and atherosclerosis, AGE-RAGE signaling pathway in diabetic complications signaling pathway were most strongly associated with lung cancer with insomnia and were more enriched, which were likely to be the potential mechanisms and pathways for the treatment of lung cancer with insomnia with the Liujunzi Tang combined with Suanzaoren Tang. This evidence concerns the gene RENBP and lung carcinoma.