Notably, the expression of S100A8, S100A9, and S100A12 in bulk liver tissue was positively correlated with ATI (S100A8: r=0.54, p=0.02; S100A9: r=0.64, p=0.004; S100A12: r=0.49, p=0.04), potentially indicating an accumulation of these cells in the liver of patients with MASLD, similar to what has been observed in a mouse model of NAFLD.32 This evidence concerns the gene S100A8 and metabolic dysfunction-associated steatotic liver disease.