This study revealed that treating with Fer-1 reversed the increase in α-SMA and Col1a1 levels, decreased E-cadherin levels, and reduced mesangial matrix expansion and collagen fiber deposition caused by TGF-β in vitro, suggesting that ferroptosis contributed to the EMT process and worsened renal fibrosis in CKD. Here, ACTA1 is linked to renal fibrosis.