To investigate the antiviralactivity of OSW-1 against SARS-CoV-2 in a physiologically relevantcell model, we generated a stable BCi-NS1.1 cell line expressing theACE2 receptor (BCi-ACE2) following infection with a replication-deficientlentivirus expressing ACE2 and subsequent neomycin selection of infectedcells.32 In addition, a control cell line(BCi-Control) was created in tandem via infection with the empty vectorcontrol lentivirus that lacks the expression of human ACE2. Here, ACE2 is linked to infection.