To evaluate the impact of SIRT3 on tumor-forming capacity in vivo, we transplanted subcutaneously SIRT3-KO and control KYSE30 cells into nude mice, then we found that decreasing SIRT3 greatly contributed to a stronger tumorigenic potential than control group as reflected by increased frequency of tumorigenic cells (Fig. 2, H–K). Here, SIRT3 is linked to neoplasm.