Overall, considering the staining intensity (scored as: 1 for weak, 2 for medium and 3 for high intensity, respectively) a lower expression, albeit not significant, of melanoma specific markers was observed on tumor tissue from R pts compared to non relapsed (NR) pts (Melan-A 1.43 ± 0.20 vs. 1.33 ± 0.66, Pmel 1.29 ± 0.52 vs. 1.67 ± 0.88, Tyrosinase 1.57 ± 0.20 vs. 1.67 ± 0.88, Ny-Eso 1 0.17 ± 0.17 vs. 1.00 ± 1.00, mean staining intensity ± SEM) (Fig. 2C). Here, MLANA is linked to melanoma.