Only Nkx2.5 was found to be significantly reduced in CMs exposed to 6 h of ischemia (0.59 ± 0.16, p < 0.05) and after 16 h of CMs reperfusion (0.54 ± 0.14, p < 0.01) (supplementary material Fig. S5). This evidence concerns the gene NKX2-5 and ischemia.