Subsequently, protein samples of CD37fl/fl and CD37−/− AML LSCs were utilized for western blot validation (The activation of phosphatidylinositol 3-kinase [PI3K]-AKT pathway was indicated by phosphorylated AKT, while the expression of Cdkn1b was determined by its translational product p27). This evidence concerns the gene CDKN1B and acute myeloid leukemia.