DAG1 and neuromuscular disease caused by qualitative or quantitative defects of alpha-dystroglycan: We analyzed different DG point-mutations linked to dystroglycanopathies, namely, L84F, T190M, I591D and C667F (Figure 1A) that were introduced in the coding sequence of murine dag1 cloned in a pEGFP-N1 vector (Pirolli et al., 2014; Palmieri et al., 2017; Signorino et al., 2018).