Scratch tests and Transwell invasion assays revealed that, when compared to the CRC stem cell group, YGS group and Gomisin B group had a significant reduction in cell migration and invasion (P < 0.01), while no substantial changes were noted in the Gomisin B + TLR4 overexpression group (Fig. 8B and C). This evidence concerns the gene TLR4 and colorectal carcinoma.