Compared with the CRC stem cell group, the protein levels of TLR4, p-IKKα/β and p-IKBα in the YGS group and the Gomisin B group showed a statistically significant reduction (P < 0.01), while no obvious change was observed in the Gomisin B + TLR4 overexpression group. This evidence concerns the gene TLR4 and colorectal carcinoma.