Here, we generated midbrain organoids using an iPSC line from a PD patient carrying the heterozygous MIRO1 p.R272Q mutation (PD or PD-MIRO1), the respective isogenic control iPSC line (correction of the MIRO1 point mutation; GC), and an iPSC line from a sex- and age-matched healthy individual (WT) (Table 1). Here, RHOT1 is linked to Parkinson disease.