Together with α‐Syn immunoreactive aggregates, progressive neuronal loss in selected brain regions, such as the SNpc, the ventral tegmental area, or the cholinergic pedunculopontine nucleus, is also a neuropathological feature of PD.[60] Yet, we did not observe any significant loss of a specific neuronal population in 3×SNCA HBO from day 30 to day 60 of differentiation (Figure S1F, Supporting Information), including the dopaminergic TH‐positive neuronal population (Figure S4E,F, Supporting Information). Here, TH is linked to Parkinson disease.