Furthermore, because MASLD is associated with an impaired suppression of hepatic glucose output (Marchesini et al., 2001), we assessed the expression of genes involved in gluconeogenesis, including glucose-6-phosphatase catalytic subunit (G6pc1) and phosphoenolpyruvate carboxykinase (Pepck). This evidence concerns the gene PCK2 and metabolic dysfunction-associated steatotic liver disease.