In addition, we found that while only approximately 47% of the CD9+ ATMs (F4/80+CD11b+CD9+) express Trem2, an overwhelming majority (~86.7%) of Trem2+ ATMs (F4/80+CD11b+Trem2+) expresses CD9 (Figure 6—figure supplement 2C), corroborating a previous finding that Trem2+ expression emerges after CD9+ ATMs over the course of obesity (Jaitin et al., 2019). This evidence concerns the gene ITGAM and obesity due to melanocortin 4 receptor deficiency.