In the 020 cell line established from a primary cancer (gingival carcinoma) that does not express TB, and in the 020G cell line established from a secondary cancer (tongue cancer) that does express TB, TGF-β expressed EMT-TFs and increased various p-EMT marker expression; however, typical EMT expression was not increased but p-EMTs were induced. The gene discussed is TGFB1; the disease is cancer.