Western blotting and in situ hybridization analyses showed that the protein level of MACF1, as well as osteogenic markers Ocn (osteocalcin) and Osterix, was decreased in the femur tissues of the older group of patients with osteoporosis aged 80–95 (vs. the group aged 60–79) and in 21-month-old mice (vs. the group aged 6 months). This evidence concerns the gene MACF1 and osteoporosis.