The objectives of this investigation are twofold: (i) to comprehensively assess OC’s regulatory effects on PAR-2 expression at both transcriptional and translational levels in established colorectal cancer models, and (ii) to systematically evaluate its downstream impact on TNF-α secretion, intracellular calcium homeostasis, and oncogenic signaling pathway activation. This evidence concerns the gene F2RL1 and colorectal cancer.