PJA1 and Anxiety: Open field testing revealed that Pja1-OE mice exhibited a significantly shorter ambulatory distance in the center zone compared to controls (Figure 7(Ac); Pja1-OE: 222.7 ± 48.4 cm, n = 9; OC: 406 ± 58.5 cm, n = 10; * p < 0.05, Mann-Whitney test), suggesting increased anxiety-like behavior.