CD4 and infection: The outgrowth kinetics from p24-normalized inputs of these WT, L56V, and N57H viruses were then evaluated over a 14-day period in mitogen-activated primary human CD4+ T cells from two independent peripheral blood mononuclear cell (PBMC) donors by measuring the amount of secreted NanoLuc in cell-free supernatants obtained 0, 2, 4, 7, 9, 11, and 14 days post-infection (Fig. 6).